Laboratorio de Ecotoxicología Acuática, Junín de los Andes, Neuquén, Argentina . likes · 2 talking about this · 1 was here. Laboratorio de. Laboratorio de Ecotoxicología Acuática, Junín de los Andes, Neuquén, Argentina . likes · 1 was here. Laboratorio de Ecotoxicología Acuática (LEA). Laboratorio de Ecotoxicología Acuática in Junín De Los Andes – Contact details, Address Map, Photos, offers, Real time Reviews and Ratings.
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Triplicate samples were collected at the upstream reach —, — and — mat the oil spill zero metersat the downstream reach 50, and m acuatifa further downstreamand m. Gene transcript expression of several cytochrome P oxidases was studied by semi-quantitative PCR in both field and laboratory. Lysosomal membrane destabilization were more closely, but not exclusively, matched with LC exposure.
This explains the similar response in liver CYP1A transcript levels at 24 and 96 h during our experiments, without dose-dependent effects.
Gill mass factor GF is affected by the E. This well-preserved low-order stream is particularly suitable for oil exposure biomarker studies since the control and the impacted areas are consecutive reaches, thus, the studied individuals belong to the same population, and the oil spill is the only cause of water quality change.
First, we explored the heavy crude oil effects on these possible biomarkers in wild juvenile rainbow trout that were caught in the La Mina stream, up and downstream of the oil spill. The only effect attributable exclusively to PST was increased phagocytic activity after 3 days and impaired feeding activity after 7 days, which curtailed toxin accumulation in digestive glands.
In this study we analyzed the suitability of different possible CYPs molecular biomarkers in O. Mussels were fed for 90 days with E. The annealing temperatures and ecotoxicologja pairs used during the duplex PCR for each gene are shown in Table I. These clear and consistent changes reaffirm CYP1A transcript expression as a good semiquantitative biomarker for crude oil exposure.
Data was complemented with laboratory exposure to water accommodated fraction WAF from the oil spill. The ecotoxiccologia obtained in wild rainbow trout could be particularly useful to evaluate other freshwater pollution, since the TPH concentration varied along the stream reach and rainbow trout was acuatixa exposed to a constant concentration of the pollution for several hours, unlike the experiments.
Hence, the response of the last ones to crude oil could be less specific but not less useful indicating some metabolic alterations. To disentangle the effect of neurotoxic vs lytic secondary metabolites, we exposed blue mussels to either a paralytic shellfish toxin PST producing Alexandrium spp. The TPH was extracted from each acuatoca carbon tetrachloride and was estimated by the analysis of the triple peak method EPA at three spectral regions: Rainbow trout Oncorhynchus mykiss is a useful model for toxicology studies because of its sensitivity to chemicals Bailey et al.
Petroleum hydrocarbons Spectrophotometric infrared.
A bp ladder Winkler was used as the molecular mass marker. The study was conducted in two m-long reaches: Anchorage Alaska, Februarypp.
Biological monitoring of marine pollutants F. Cytochrome P CYP in fish. Auatica were diluted with ultra-pure water to adjust to a concentration of 2. However, there are few studies on the nature of PXR ligands in fish Ekins et al.
For the experiments, juvenile rainbow trout 3. As far as we know, this is the first ecotoxicological study on the oil spill in La Mina stream, and one of the few related studies in Patagonia. Editado por TW Parsons, Bariloche, pp. The upstream reach is up of a 1. Cytochrome P CYP gene expressions were studied by semi-quantitative polymerase chain reaction PCR to assess oil pollution using liver and gills of juvenile rainbow trout Oncorhynchus mykiss sampled from La Mina stream Bariloche, Argentinaup and downstream of a discharge of petroleum spill from an unproductive wellbore.
Informe, Buenos Aires, Argentina, 64 pp. Julieta Soledad Acuatoca Anna.
In vitro effects of endosulfan-based insecticides on mammalian sperm.
Comparison of rainbow trout and mammalian cytochrome P enzymes: The evolution of farnesoid X, vitamin D, and pregnane X receptors: Phagocytic activity is higher in EG mussels than in SV ones under all conditions. TPH concentration in sediments was 0.
Oil exposure induced gill and liver Ecotpxicologia transcription 1. The maximum induction of CYP1A transcript expression has been reported to start eight to sixteen hours after exposure Minura et al.
Departamento de Química Biológica
The study of mRNA expression patterns of multiple CYP genes in rainbow trout gills and liver would be extended in order to evaluate their use for monitoring programs of crude oil pollution and remediation actions on Patagonian freshwater ecosystems. Our results show that the total hemocyte number decreases while pollution load increases along the river course for both, EG and SV mussels.
Thus, anomalous expression of acutaica CYP2 could impact on steroid and fatty acid homeostasis in rainbow trout e. The spills converge into a unique source point of pollution, with liters of crude oil per day entering the stream in February The TPH was 5. Fish were killed and dissected as described above for field samples. The climate is cold-temperate, with a mean annual temperature of 8.