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TM 12 Genetic engineering in action 1 (Biologi Molekuler ).pptx – Download as .ppt /.pptx), PDF File .pdf), Text File .txt) or view presentation slides online . Perkembangan Biologi Molekuler dan Pembelajarannya. Sutiman B. Sumitro. Laboratorium Biologi Sel dan Molekuler. Fakultas MIPA. Universitas Brawijaya. Slide 1 TATAP MUKA 13 Genetic engineering in action (2) 1 Learning Outcome ( LO) LO menjelaskan tanaman transgenik LO menjelaskan hewan.

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The technique requires a mechanical micromanipulator and a microscope, and plenty of practice! The method is summarised in Fig. Sel-sel ES yang mengandung transgen yang disuntikkan ke dalam sel-sel ES dari embrio yang lain, di mana mereka dimasukkan ke dalam massa sel. Pada inti, kode gen ditranskripsikan pada RNA. An organism in which both copies of the gene are identical – that is, alide the same allele – is said to be homozygous for that gene.

When the telomere becomes too short, bioligi chromosome reaches a “critical length” and can no longer replicate. These specify either shooty or rooty tumours. Addition is determined by the activity of telomerase.

Daerah biolpgi diperlukan untuk adsorpsi ke sel inang. Another example of this type of variation is where the embryo is generated from two distinct individuals, as is the case when embryonic stem cells are used. Each time a cell divides, an average person loses 30 to base pairs from the ends of that cells telomeres.

They are small 46 kb and can therefore accommodate cloned DNA fragments up to some 47 kb in length. Untuk men-downloadsilahkan rekomendasi presentasi ini kepada teman-teman Anda dalam jaringan sosial. Protecting the vital DNA molecule from being copied out of synch, these telomeres provide a kind of buffer zone where asynchronous replication errors that are inevitable will not result in any of the vital DNA sequences being lost.

In naming plasmids, p is used to designate plasmid, and this is usually followed by the initials of the worker s who isolated or constructed the plasmid. Process of copying DNA is called replication. Dua jenis utama plasmid Ti yang ditemukan biasanya dapat dicirikan atas dasar gen penyandi octopine dan nopaline.


Dolly died at 6 years old from progressive lung disease symptom of old age ChromosomeTelomeresTelomeresTelomere Length DeclinesTelomere length in base pairs human white blood cells More on Molekler and CloningHow many failures will it take to produce a human Dollys experimenters used cloned embryos to produce one sheep, meaning slid.

Such studies provided the essential background information on which genetic engineering is based. The hatched region shows the two fragments that were removed from pBR to generate pAT In addition to GM crops, transgenic plants also have the potential to make a significant impact on the biotechnology of therapeutic protein production.

On infection, the T-DNA becomes integrated into the plant cell genome and is therefore a possible avenue for the introduction of foreign DNA into the plant genome. To use this website, you must agree to our Privacy Policyincluding cookie policy.

TM 13 APLIKASI MANIPULASI GEN 2 (Biologi Molekuler ).pptx

Telomeres function by preventing chromosomes from losing base pair sequences at their ends. Terms marked TM are trademarks of Stratagene.

Ltm 1 Aplikasi Biologi Molekuler Documents. Numbers may be used to classify the particular isolate. This moleluler some kb in size and also carries the gene lside octopine or nopaline synthesis.

These act as cohesive or sticky ends, which enable circularisation of the genome following infection. Each time our cells duplicate themselves, a small portion of the DNA molecule is lost and not copied. Vektor yang digunakan dalam sel eukariotik23LO Happens in all types of cells, prokaryotes and eukaryotes.

TM 13 APLIKASI MANIPULASI GEN 2 (Biologi Molekuler 2014).pptx

Pendekatan ini memungkinkan kloning fragmen DNA yang lebih besar. Nonconjugative plasmids are not self-transmissible, but may be mobilised by a conjugation-proficient plasmid if their mob region is functional.

As -carotene is a precursor of vitamin A, increasing the amount available by engineering rice in this way should help to alleviate some of the problems of vitamin A deficiency. Pada kedua ujung genom linier terdapat daerah untai tunggal 12 basa yang dikenal sebagai situs cos cos site. It is then replicated with the chromosomal DNA and passed on to daughter cells in a stable form.


The one disadvantage of the Ti system is that it does not normally infect monocotyledonous monocot plants such as cereals and grasses. The telomere can reach a length of 15, base pairs. Biollogi phages are those that exhibit a lytic life cycle only. Salah satu turunan transgenik mengekspresi transgen pada jaringan otak tertentu Gambar. Horizontal gene transfer — donor contributes giologi of genome to recipient that are not descendants; three types: ANDi is particularly significant in that he opens up the possibility of a near-human model organism for the evaluation of disease and therapy.

Firstly, the arrangement of genes on the genome will determine which parts can be removed or replaced for the addition of exogenous DNA. Some unique restriction sites are given. Kedua plasmid saling melengkapi secara trans untuk s,ide transfer DNA kloning ke moolekuler genom tanaman. Conversely, later studies show that VEGF receptor-1 can interact with signal-transducing proteins and generate a mitogenic signal.

Published by Julian Powers Modified 4 months ago. Omlekuler is now part of Monsanto, having been stretched too far by development of the Flavr Savr.

Konstruk gen ini disuntikkan ke pronukleus jantan dan tikus transgenik yang diproduksi. The theory is simple — if plants can be made herbicideresistant,then weeds can be treated with a broad-spectrum herbicide without the crop plant being affected. It will certainly be interesting to follow the developments in transgenic technology over the next few years.